Department of Pediatrics, British Columbia Children's Hospital and Child and Family Research Institute, University of British Columbia, Vancouver, BC, Canada, V6H 3V4.
Clin Biochem. 2012 Jan;45(1-2):178-80. doi: 10.1016/j.clinbiochem.2011.10.023. Epub 2011 Nov 7.
To investigate the effect of hemolysis and protease inhibitors on acylated ghrelin (AG) concentrations measured with a double antibody enzyme immunometric assay that uses an acetylcholinesterase (AChE)-Fab' conjugate.
Samples were hemolysed or treated with PHMB (p-hydroxymercuribenzoate), PMSF (phenylmethanesulfonylfluoride) or AEBSF (4-(2-Aminoethyl) benzenesulfonyl fluoride) to prevent AG degradation.
Hemolysis decreased AG concentrations. PHMB or PMSF did not affect the assay. The standard curve was abolished by AEBSF but rescued by addition of a washing step prior to the AChE-Fab' conjugate.
Hemolysis and AEBSF may affect AG determination.
研究使用乙酰胆碱酯酶（AChE）-Fab' 缀合物的双抗体酶免疫测定法测量酰化生长素释放肽（AG）浓度时，溶血和蛋白酶抑制剂的影响。
对样品进行溶血处理或用 PHMB（对羟基汞苯甲酸）、PMSF（苯甲基磺酰氟）或 AEBSF（4-（2-氨基乙基）苯磺酰氟）处理以防止 AG 降解。
溶血降低了 AG 浓度。PHMB 或 PMSF 不影响测定。AEBSF 使标准曲线失效，但在添加 AChE-Fab' 缀合物之前增加洗涤步骤可使其恢复。
溶血和 AEBSF 可能会影响 AG 的测定。
