Pathologisches Institut der Ludwig-Maximilians-Universität München, Thalkirchner Straße 36, D-80337 München, Germany.
Exp Mol Pathol. 2012 Feb;92(1):111-7. doi: 10.1016/j.yexmp.2011.10.010. Epub 2011 Nov 10.
Aldehyde dehydrogenase-1 (ALDH1) is involved in the regulation of cell proliferation and differentiation. Moreover, it is a marker for cancer stem cells (CSC). As CSCs were shown to be the driving force of tumor progression and metastases we suspected that the expression of ALDH1 correlated with the prognostic 5 year survival of colorectal cancer.
ALDH1 expression was analyzed in a highly stratified collective of 186 T3 N0 M0 G2 primary colorectal cancer specimens applying immunohistochemistry. For the analysis a scoring system for the expression of ALDH1 was developed that was aided by the pattern of the subcellular expression of beta-catenin which is a well known indicator for colorectal CSCs.
First, ALDH1 expression could be assigned to two groups which correlated with the absence or presence of nuclear beta-catenin expression. Second, ALDH1 group 2 expression patterning correlated highly significantly with low long term survival (p=0.010) of patients with T3 N0 M0 G2 colorectal cancer. This correlation was found univariately and when applying the multivariate Cox-model.
ALDH1 expression pattern is an independent prognostic marker for survival of T3 N0 M0 G2 colorectal cancer patients.
醛脱氢酶 1（ALDH1）参与细胞增殖和分化的调节。此外，它是癌症干细胞（CSC）的标志物。由于 CSC 被认为是肿瘤进展和转移的驱动力，我们怀疑 ALDH1 的表达与结直肠癌的预后 5 年生存率相关。
应用免疫组织化学分析了 186 例高度分层的 T3N0M0G2 原发性结直肠癌标本中 ALDH1 的表达。为了进行分析，开发了一种用于 ALDH1 表达的评分系统，该系统得到了β-连环蛋白亚细胞表达模式的辅助，β-连环蛋白是结直肠 CSC 的已知标志物。
首先，ALDH1 的表达可以分为两组，这与核β-连环蛋白表达的存在或不存在相关。其次，ALDH1 组 2 表达模式与 T3N0M0G2 结直肠癌患者的低长期生存率高度显著相关（p=0.010）。这种相关性在单变量和应用多变量 Cox 模型时均存在。
ALDH1 表达模式是 T3N0M0G2 结直肠癌患者生存的独立预后标志物。
