The Department of Ophthalmology, The Second Hospital of Hebei Medical University, Heping Road No.125, Shijiazhuang, 050000, Hebei Province, China.
Graefes Arch Clin Exp Ophthalmol. 2012 Mar;250(3):341-8. doi: 10.1007/s00417-011-1854-y. Epub 2011 Nov 19.
The study aims to determine the changes in the biomechanical properties of the anterior and extreme posterior portions of experimental near-sighted eyes by examining the mechanical behavior of guinea pig scleral desmocytes, thus finding a new approach to the pathogenesis of myopia and their corresponding therapies.
Guinea pigs (2 weeks old) were numbered and assigned into three groups (A, B, and C) with ten guinea pigs each. Concave lens-induced myopic (LIM) animal models were prepared via the out-of-focus method. The other eye in the same guinea pig served as the self-control (SC) group. After modeling groups A, B, and C for 6, 15, and 30 days respectively, the lenses from the guinea pigs in the experimental group were removed. The scleral fibroblasts in each group were cultured, and passaged twice in vitro. The micropipette aspiration technique coupled with a viscoelastic solid model was utilized to investigate the viscoelastic properties of the scleral fibroblasts in normal and myopic guinea pigs. The mechanical behavior of the scleral desmocytes of the LIM and SC groups were compared.
The mechanical behavior of the scleral desmocytes was compared between the LIM and SC groups. The Young's modulus at equilibrium and the apparent cellular viscosity of the anterior portion of the sclera in the LIM group at 6 days and 15 days after myopic induction were not significantly different from that of the SC group (P < 0.05). However, the results for the anterior portions of the sclera in the LIM group at 30 days were significantly higher than those of the LIM group at 6 and 15 days, as well as those in the SC group (P < 0.05). The Young's modulus at equilibrium and the apparent cellular viscosity of the extreme posterior portions of the sclera in the LIM group at 6 days after myopic induction not significantly from those of the SC group (P < 0.05). However, the results for the extreme posterior portions of the sclera in the LIM group after 15 days and 30 days were significantly higher than those in the LIM group at 6 days and the SC group (P < 0.05).
The Young's modulus at equilibrium or apparent cellular viscosity of all the anterior portions of the sclera in the LIM group were longer than those in the SC group at 30 days after the induction, and the results for all the extreme posterior portions of the LIM group were larger than those of the SC group on the 15th and 30th day. Therefore, the Young's modulus and apparent viscosity of the anterior and extreme posterior portions of the sclera changed on the 15th and 30th day after induction respectively.
本研究旨在通过观察豚鼠巩膜细胞外基质细胞的力学行为，探讨实验性近视眼前极和后极生物力学特性的变化，从而为近视的发病机制及其相应的治疗方法提供新的思路。
将 2 周龄豚鼠编号并分为三组（A、B、C 组），每组 10 只。采用离焦法建立近视诱导动物模型。同一只豚鼠的另一只眼作为自身对照（SC）组。分别对 A、B、C 组建模 6、15、30 天后，取出实验组豚鼠的晶状体。培养各组巩膜成纤维细胞，体外传代 2 代。采用微管吸吮技术与粘弹性固体模型相结合的方法，研究正常和近视豚鼠巩膜成纤维细胞的粘弹性特性。比较 LIM 组和 SC 组巩膜细胞外基质细胞的力学行为。
比较 LIM 组和 SC 组巩膜细胞外基质细胞的力学行为。近视诱导后 6 天和 15 天，LIM 组前极巩膜的平衡杨氏模量和表观细胞黏度与 SC 组无显著差异（P>0.05）。然而，LIM 组前极巩膜在 30 天时的结果明显高于 6 天时和 15 天时，也明显高于 SC 组（P<0.05）。近视诱导后 6 天，LIM 组后极巩膜的平衡杨氏模量和表观细胞黏度与 SC 组无显著差异（P>0.05）。然而，15 天和 30 天后，LIM 组后极巩膜的结果明显高于 6 天时的 LIM 组和 SC 组（P<0.05）。
诱导后 30 天，LIM 组前极巩膜的平衡杨氏模量或表观细胞黏度均长于 SC 组，15 天和 30 天，LIM 组后极巩膜的所有结果均大于 SC 组。因此，巩膜前极和后极的杨氏模量和表观黏度分别在诱导后 15 天和 30 天发生变化。
