Snejdrlova Michaela, Kalvach Zdenek, Topinkova Eva, Vrablik Michal, Prochazkova Renata, Kvasilova Marie, Lanska Vera, Zlatohlavek Lukas, Prusikova Martina, Ceska Richard
3rd Department of Internal Medicine, 1st Faculty of Medicine, Charles University Prague, Czech Republic.
Neuro Endocrinol Lett. 2011;32 Suppl 2:51-4.
Life expectancy is determined by a combination of genetic predisposition (25%) and environmental influences (75%). Nevertheless a stronger genetic influence is anticipated in long-living individuals. Apolipoprotein E (APOE) gene belongs among the most studied candidate genes of longevity. We evaluated the relation of APOE polymorphism and fitness status in the elderly.
We examined a total number of 128 subjects, over 80 years of age. Using a battery of functional tests their fitness status was assessed and the subjects were stratified into 5 functional categories according to Spirduso´s classification. Biochemistry analysis was performed by enzymatic method using automated analyzers. APOE gene polymorphism was analysed performed using PCR-RFLP.
APOE4 allele carriers had significantly worse fitness status compared to non-carriers (p=0.025). Multiple logistic regression analysis showed the APOE4 carriers had higher risk (p=0.05) of functional unfitness compared to APOE2/E3 individuals.
APOE gene polymorphism seems be an important genetic contributor to frailty development in the elderly. While APOE2 carriers tend to remain functionally fit till higher age, the functional status of APOE4 carriers deteriorates more rapidly.
预期寿命由遗传易感性（约25%）和环境影响（约75%）共同决定。然而，预计长寿个体中遗传影响更强。载脂蛋白E（APOE）基因是研究最多的长寿候选基因之一。我们评估了老年人中APOE基因多态性与健康状况的关系。
我们检查了总共128名80岁以上的受试者。通过一系列功能测试评估他们的健康状况，并根据斯皮德苏的分类将受试者分为5个功能类别。使用自动分析仪通过酶法进行生化分析。使用PCR-RFLP分析APOE基因多态性。
与非携带者相比，APOE4等位基因携带者的健康状况明显更差（p = 0.025）。多元逻辑回归分析显示，与APOE2/E3个体相比，APOE4携带者功能不健全的风险更高（p = 0.05）。
APOE基因多态性似乎是老年人虚弱发展的一个重要遗传因素。虽然APOE2携带者往往在较高年龄时仍保持功能健康，但APOE4携带者的功能状态恶化得更快。
