Genç Oktay, Büyüktanır Ozlem, Yurdusev Nevzat
Faculty of Veterinary Medicine, Department of Microbiology, Ondokuz Mayıs University, Samsun, Turkey.
Trop Anim Health Prod. 2012 Feb;44(2):213-5. doi: 10.1007/s11250-011-0019-7. Epub 2011 Nov 21.
A non-enzymatic rapid immunofiltration assay (NERIFA) was developed as an alternative field test for rapid detection of anti-Brucella antibody in bovine and ovine sera. The assay was based on Brucella abortus lipopolysaccharide as diagnostic antigen and colloidal gold particle-protein G conjugate as detection reagent. Its diagnostic performance was evaluated using undiluted well-defined positive and negative serum samples in comparison with Rose Bengal test (RBT), complement fixation test (CFT) and a commercial and an in-house indirect enzyme-linked immunosorbent assay (ELISA). A perfect test agreement was found between NERIFA and ELISAs by kappa statistics. In addition, McNemar's analysis of the results showed that the RBT for bovine sera and the CFT for ovine sera were found significantly less performant than indirect ELISAs and NERIFA. The results of the present study indicated that the NERIFA could be considered as a simple, rapid, and accurate field test for screening of ovine and bovine brucellosis. Therefore, this test constitutes a high potential to be used as an alternative model particularly in brucellosis prevalent tropical and subtropical geographical areas.
开发了一种非酶促快速免疫过滤测定法（NERIFA），作为一种用于快速检测牛和羊血清中抗布鲁氏菌抗体的替代现场检测方法。该测定法以流产布鲁氏菌脂多糖作为诊断抗原，胶体金颗粒-蛋白G缀合物作为检测试剂。与玫瑰红试验（RBT）、补体结合试验（CFT）以及一种商业和一种自制间接酶联免疫吸附测定法（ELISA）相比，使用未稀释的明确阳性和阴性血清样本对其诊断性能进行了评估。通过kappa统计发现NERIFA与ELISA之间具有完美的检测一致性。此外，对结果的McNemar分析表明，牛血清的RBT和羊血清的CFT的性能明显低于间接ELISA和NERIFA。本研究结果表明，NERIFA可被视为一种用于筛查羊和牛布鲁氏菌病的简单、快速且准确的现场检测方法。因此，该检测方法具有很高的潜力，可作为一种替代模型，特别是在布鲁氏菌病流行的热带和亚热带地理区域。
