Garriga Damià, Vives-Adrián Laia, Buxaderas Mònica, Ferreira-da-Silva Frederico, Almeida Bruno, Macedo-Ribeiro Sandra, Pereira Pedro José Barbosa, Verdaguer Núria
Institut de Biologia Molecular de Barcelona, CSIC, Parc Científic de Barcelona, Baldiri i Reixac 10, 08028 Barcelona, Spain.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1224-7. doi: 10.1107/S1744309111026261. Epub 2011 Sep 29.
The Picornaviridae family contains a large number of human pathogens such as rhinovirus, poliovirus and hepatitis A virus (HAV). Hepatitis A is an infectious disease that causes liver inflammation. It is highly endemic in developing countries with poor sanitation, where infections often occur in children. As in other picornaviruses, the genome of HAV contains one open reading frame encoding a single polyprotein that is subsequently processed by viral proteinases to originate mature viral proteins during and after the translation process. In the polyprotein, the N-terminal P1 region generates the four capsid proteins, while the C-terminal P2 and P3 regions contain the enzymes, precursors and accessory proteins essential for polyprotein processing and virus replication. Here, the first crystals of protein 2AB of HAV are reported. The crystals belonged to space group P4(1) or P4(3), with unit-cell parameters a = b = 90.42, c = 73.43 Å, and contained two molecules in the asymmetric unit. Native and selenomethionine-derivative crystals diffracted to 2.7 and 3.2 Å resolution, respectively.
小核糖核酸病毒科包含大量人类病原体，如鼻病毒、脊髓灰质炎病毒和甲型肝炎病毒（HAV）。甲型肝炎是一种引起肝脏炎症的传染病。在卫生条件差的发展中国家，它具有高度地方性流行，感染通常发生在儿童中。与其他小核糖核酸病毒一样，HAV的基因组包含一个开放阅读框，编码一个单一的多聚蛋白，该多聚蛋白随后在翻译过程中及翻译后由病毒蛋白酶加工，产生成熟的病毒蛋白。在多聚蛋白中，N端的P1区域产生四种衣壳蛋白，而C端的P2和P3区域包含多聚蛋白加工和病毒复制所必需的酶、前体和辅助蛋白。在此，报道了HAV的2AB蛋白的首批晶体。这些晶体属于空间群P4(1)或P4(3)，晶胞参数a = b = 90.42，c = 73.43 Å，不对称单位中包含两个分子。天然晶体和硒代甲硫氨酸衍生晶体分别衍射至2.7 Å和3.2 Å分辨率。
