Zhang Jinxiu, Yang Xue, Shen Yuequan, Long Jiafu
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1443-7. doi: 10.1107/S174430911103689X. Epub 2011 Oct 27.
The L27 (LIN-2/LIN-7) domain is a protein-protein interaction module capable of assembling proteins into biologically important complexes. Pals1 contains two L27 domains: the first, L27N, interacts with PATJ, and the second, L27C, interacts with MALS, forming a tripartite complex that plays a crucial role in the establishment and maintenance of cell polarity. To provide a better understanding of the mechanism of assembly of this tripartite complex, four different L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) constructs were cloned, expressed, purified and crystallized. Crystals of tripartite complex 1 of L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) diffracted to 2.05 Å resolution. These crystals belonged to either space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 145.2, c = 202.5 Å. Assuming the presence of four molecules in the asymmetric unit, a Matthews coefficient of 2.69 Å(3) Da(-1) was calculated, corresponding to a solvent content of 54.25%.
L27（LIN-2/LIN-7）结构域是一种蛋白质-蛋白质相互作用模块，能够将蛋白质组装成具有生物学重要性的复合物。Pals1包含两个L27结构域：第一个是L27N，与PATJ相互作用；第二个是L27C，与MALS相互作用，形成一个三方复合物，该复合物在细胞极性的建立和维持中起关键作用。为了更好地理解这个三方复合物的组装机制，克隆、表达、纯化并结晶了四种不同的L27（PATJ）-（L27N，L27C）（Pals1）-L27（MALS）构建体。L27（PATJ）-（L27N，L27C）（Pals1）-L27（MALS）三方复合物1的晶体衍射分辨率达到2.05 Å。这些晶体属于空间群P6(1)22或P6(5)22，晶胞参数a = b = 145.2，c = 202.5 Å。假设不对称单位中存在四个分子，计算出的马修斯系数为2.69 Å(3) Da(-1)，对应溶剂含量为54.25%。
