Hatami Mehdi, Farhadi Khalil
Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran.
Biomed Chromatogr. 2012 Jul;26(7):875-80. doi: 10.1002/bmc.1746. Epub 2011 Nov 20.
A hollow fiber liquid-phase microextraction technique coupled with high-performance liquid chromatography with fluorescence detection was employed for determination and evaluation of the binding characteristics of drugs to bovine serum albumin (BSA). Enantiomers of guaifenesin (an expectorant drug) were investigated as a model system. After optimization of some influencing parameters on microextraction, the proposed method was used for calculation of the target drug distribution coefficient between n-octanol and the buffer solution as well as study of drug-BSA binding in physiological conditions. The developed method shows a new, improved and simple procedure for determination of free drug concentration in biological fluids and the extent of drug-protein binding.
采用中空纤维液相微萃取技术结合高效液相色谱-荧光检测法，用于测定和评估药物与牛血清白蛋白（BSA）的结合特性。以愈创甘油醚（一种祛痰药）的对映体作为模型体系进行研究。在对微萃取的一些影响参数进行优化后，该方法用于计算目标药物在正辛醇和缓冲溶液之间的分配系数，以及研究生理条件下药物与BSA的结合情况。所建立的方法为测定生物流体中的游离药物浓度和药物-蛋白质结合程度提供了一种新的、改进的且简单的程序。
