Barbés C, Sánchez J, Yebra M J, Robert-Geró M, Hardisson C
Departamento de Microbiologia, Facultad de Medicinia, Universidad de Oviedo, Spain.
FEMS Microbiol Lett. 1990 Jun 1;57(3):239-43.
Sinefungin is a naturally occurring nucleoside isolated from cultures of Streptomyces griseolus and S. incarnatus. It is structurally related to S-adenosyl-methionine (SAM) and S-adenosyl-L-homocysteine (SAH). Its effect and level of action on prokaryotes has not been studied with the same detail as with eukaryotic cells. In this report we describe the effect of sinefungin and SAH on several Streptomyces methyltransferases (DNA and protein MTases) and on other bacterial DNA-MTases. Protein MTases are resistant to sinefungin, whereas DNA-MTases are inhibited. Adenine MTases however, seem more sensitive to this analogue than cytosine MTases.
西奈芬净是一种从灰链霉菌和肉色链霉菌培养物中分离出的天然核苷。它在结构上与S-腺苷甲硫氨酸（SAM）和S-腺苷-L-高半胱氨酸（SAH）相关。其对原核生物的作用效果和作用水平尚未像对真核细胞那样进行详细研究。在本报告中，我们描述了西奈芬净和SAH对几种链霉菌甲基转移酶（DNA和蛋白质甲基转移酶）以及其他细菌DNA甲基转移酶的影响。蛋白质甲基转移酶对西奈芬净具有抗性，而DNA甲基转移酶则受到抑制。然而，腺嘌呤甲基转移酶似乎比胞嘧啶甲基转移酶对这种类似物更敏感。
