Matsumoto Akihiro, Hatta Toshihisa, Ono Atsuki, Hashimoto Ryuju, Otani Hiroki
Department of Developmental Biology, Shimane University, Izumo Department of Anatomy I, Kanazawa Medical University, Kahoku-gun, Japan.
Congenit Anom (Kyoto). 2011 Dec;51(4):183-6. doi: 10.1111/j.1741-4520.2011.00337.x.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic cytokine that has neurotrophic and neuroprotective functions. However, its function in the mid- to late-gestational fetus remains unclear. We used enzyme-linked immunosorbent assay to analyze GM-CSF levels in cerebrospinal fluid (CSF), serum, and amniotic fluid of mouse fetuses. We also examined GM-CSF and receptor α (GM-CSFRα) levels in the fetal brain, liver, and placenta. GM-CSF peaked between embryonic day (E) 14 and E15 in the CSF. GM-CSF level was higher in the fetal serum than in the dam serum on E13 and decreased thereafter. GM-CSF and GM-CSFRα levels peaked between E13 and E15 in the brain. These results suggest that GM-CSF plays stage- and organ-specific roles in fetal development.
粒细胞-巨噬细胞集落刺激因子（GM-CSF）是一种具有神经营养和神经保护功能的造血细胞因子。然而，其在妊娠中后期胎儿中的功能仍不清楚。我们使用酶联免疫吸附测定法分析了小鼠胎儿脑脊液（CSF）、血清和羊水中的GM-CSF水平。我们还检测了胎儿脑、肝和胎盘中GM-CSF及其受体α（GM-CSFRα）的水平。GM-CSF在CSF中于胚胎第（E）14天至E15天达到峰值。在E13时，胎儿血清中的GM-CSF水平高于母鼠血清，此后下降。GM-CSF和GM-CSFRα水平在脑中于E13至E15天达到峰值。这些结果表明，GM-CSF在胎儿发育中发挥着阶段和器官特异性作用。
