增殖性玻璃体视网膜前膜中自分泌运动因子和酰基甘油激酶的表达。

Expression of autotaxin and acylglycerol kinase in proliferative vitreoretinal epiretinal membranes.

机构信息

Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

出版信息

Acta Ophthalmol. 2012 Mar;90(2):e84-9. doi: 10.1111/j.1755-3768.2011.02303.x. Epub 2011 Nov 22.

DOI:10.1111/j.1755-3768.2011.02303.x

PMID:22103573

Abstract

PURPOSE

Lysophosphatidic acid (LPA)/LPA(1) receptor pathway is involved in inflammation, angiogenesis and fibrosis. This study was conducted to analyse the expression of LPA-producing enzymes, autotaxin (ATX) and acylglycerol kinase (AGK) and LPA(1) receptor, in proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR) epiretinal membranes.

METHODS

Nine active and 13 inactive membranes from patients with PDR and 21 membranes from patients with PVR were studied by immunohistochemistry.

RESULTS

In PDR membranes, vascular endothelial cells expressed ATX and AGK in 16 and 19 membranes, respectively. Stromal cells expressed ATX and AGK in 19 and 22 membranes, respectively. Immunoreactivity for LPA(1) receptor was noted in vascular endothelial cells and stromal cells in the five membranes stained for LPA(1) receptor. Numbers of blood vessels and stromal cells expressing CD34, ATX and AGK were significantly higher in active membranes than in inactive membranes. Significant correlations were detected between number of blood vessels expressing the panendothelial cell marker CD34 and number of blood vessels and stromal cells expressing ATX and AGK. In PVR membranes, spindle-shaped myofibroblasts expressing α-smooth muscle actin co-expressed ATX, AGK and LPA(1) receptor.

CONCLUSIONS

The LPA/LPA(1) receptor pathway may be involved in inflammatory, angiogenic and fibrotic responses in proliferative vitreoretinal disorders.

摘要

目的

溶血磷脂酸（LPA）/LPA（1）受体途径参与炎症、血管生成和纤维化。本研究旨在分析增殖性糖尿病性视网膜病变（PDR）和增殖性玻璃体视网膜病变（PVR）视网膜前膜中产生 LPA 的酶、自分泌运动因子（ATX）和酰基甘油激酶（AGK）以及 LPA（1）受体的表达。

方法

通过免疫组织化学方法研究了 9 例活跃性和 13 例非活跃性 PDR 患者以及 21 例 PVR 患者的视网膜前膜。

结果

在 PDR 视网膜前膜中，血管内皮细胞在 16 例和 19 例中分别表达 ATX 和 AGK。基质细胞在 19 例和 22 例中分别表达 ATX 和 AGK。在 5 例 LPA（1）受体染色的视网膜前膜中，血管内皮细胞和基质细胞均有 LPA（1）受体的免疫反应性。在活跃性视网膜前膜中，表达 CD34、ATX 和 AGK 的血管和基质细胞数量明显高于非活跃性视网膜前膜。表达血管内皮细胞标志物 CD34 的血管数量与表达 ATX 和 AGK 的血管和基质细胞数量之间存在显著相关性。在 PVR 视网膜前膜中，表达α-平滑肌肌动蛋白的梭形肌成纤维细胞共表达 ATX、AGK 和 LPA（1）受体。