Weicker H, Bieger W, Huster H
Biochim Biophys Acta. 1979 Mar 7;583(2):189-95. doi: 10.1016/0304-4165(79)90426-4.
The incubation of ghosts derived from human Rhesus-positive red cells with IgG-anti-Rhesus-D inhibited the K+-sensitive p-nitrophenylphosphatase activity. This enzyme has a partial function in the (Na+ + K+)-ATPase system related to the phosphorylation step, which is important for active potassium transport through the red cell membrane. The specificity of the impairment by the antigen-antibody reaction in the Rhesus-D system was proved by the following controls. Ghosts obtained from Rhesus-negative red cells incubated by IgG-anti-Rhesus-D and those of Rhesus-positive red cells treated with non-immune serum did not show any reduction of the K+-p-nitrophenylphosphatase activity. The ghost preparation with lanthanum carried out after hypotonic hemolysis of the washed red cells in 2 mM LaCl3 at pH 6 was the most suitable procedure to explore this topic in comparison to other techniques for preparing ghosts of red cells.
用人恒河猴阳性红细胞衍生的血影与IgG抗D抗体孵育，可抑制钾离子敏感性对硝基苯磷酸酶的活性。该酶在与磷酸化步骤相关的（钠+钾）-ATP酶系统中具有部分功能，这对于钾离子通过红细胞膜的主动转运很重要。恒河猴-D系统中抗原-抗体反应造成损伤的特异性通过以下对照得以证实。用IgG抗D抗体孵育恒河猴阴性红细胞得到的血影以及用非免疫血清处理的恒河猴阳性红细胞血影，其钾离子对硝基苯磷酸酶活性均未出现任何降低。与其他制备红细胞血影的技术相比，在pH值为6的2 mM氯化镧中对洗涤后的红细胞进行低渗溶血后用镧制备血影，是探索该主题的最合适方法。
