National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Bioresour Technol. 2012 Jan;104:466-72. doi: 10.1016/j.biortech.2011.10.049. Epub 2011 Oct 31.
Application of combined ultrafiltration (UF) and nanofiltration (NF) was examined to recycle cellulase and concentrate glucose present in lignocellulosic hydrolyzate. With PES10 membrane operated at 25.6 l/m(2) h, 73.9% of cellulase protein present in the hydrolyzate suspension could be recovered while allowing free transmission of glucose. The permeate obtained from UF was then concentrated by NF. With NF270 membrane operated at 13.3 l/m(2) h, the glucose concentration in the ultrafiltered hydrolyzate increased from 30.2 to 110.2 g/l. Recycling cellulase by UF could reduce the hydrolysis cost of lignocellulosic feedstock, while concentrating glucose by NF could improve the fermentation efficiency of lignocellulosic hydrolyzate and lower the separation and purification cost of fermentative product. Therefore, the use of UF and NF for treating lignocellulosic hydrolyzate could be a promising approach in fermentative production of bioproducts and biofuels using lignocellulosic feedstock as substrate.
联合使用超滤 (UF) 和纳滤 (NF) 来回收纤维素酶并浓缩木质纤维素水解物中存在的葡萄糖。在 PES10 膜下，操作压力为 25.6 l/m(2) h，纤维素酶蛋白在水解悬浮液中 73.9%可被回收，同时允许葡萄糖自由传输。超滤得到的渗透物随后用 NF 进行浓缩。在 NF270 膜下，操作压力为 13.3 l/m(2) h，超滤水解物中的葡萄糖浓度从 30.2 增加到 110.2 g/l。使用 UF 回收纤维素酶可以降低木质纤维素原料的水解成本，而使用 NF 浓缩葡萄糖可以提高木质纤维素水解物的发酵效率，降低发酵产物的分离和纯化成本。因此，使用 UF 和 NF 处理木质纤维素水解物可能是一种很有前途的方法，可用于以木质纤维素为底物发酵生产生物制品和生物燃料。
