Jücker M, Schaadt M, Diehl V, Poppema S, Jones D, Tesch H
Genetisches Labor Med. Klinik I, Universität Köln, Cologne, FRG.
Hematol Oncol. 1990 Jul-Aug;8(4):191-204. doi: 10.1002/hon.2900080404.
The expression of 20 proto-oncogenes was analysed by Northern blotting in four cell lines derived from patients with Hodgkin's disease (L428, L540, CO and DEV) and compared to lymphoid and myeloid leukemia cell lines and normal hematopoietic cells. Expression of the proto-oncogenes c-myc, p53, c-jun, pim-1, lck, c-syn, c-raf and N-ras were detected in Hodgkin's disease derived cell lines and in normal hematopoietic cells. Transcripts of the proto-oncogene c-met were detected in the Hodgkin's derived cell lines L428 and L540 but not in the lymphoid or myeloid leukemia cell lines or in tonsil cells, peripheral blood mononuclear cells and granulocytes. Expression of the proto-oncogenes N-myc and lck were observed in the Hodgkin's derived cell line CO which express T cell receptor genes and in the T cell lines JM and CEM. L428 cells and CO cells expressed aberrant transcripts of the c-fes proto-oncogene. Thus Hodgkin's disease derived cell lines are heterogeneous in their expression pattern of proto-oncogenes, expressing normal and aberrant transcripts of proto-oncogenes which are not found in untransformed hematopoietic cells.
通过Northern印迹法分析了源自霍奇金病患者的四种细胞系（L428、L540、CO和DEV）中20种原癌基因的表达，并与淋巴细胞和髓细胞白血病细胞系以及正常造血细胞进行了比较。在源自霍奇金病的细胞系和正常造血细胞中检测到了原癌基因c-myc、p53、c-jun、pim-1、lck、c-syn、c-raf和N-ras的表达。在源自霍奇金病的细胞系L428和L540中检测到了原癌基因c-met的转录本，但在淋巴细胞或髓细胞白血病细胞系、扁桃体细胞、外周血单个核细胞和粒细胞中未检测到。在表达T细胞受体基因的源自霍奇金病的细胞系CO以及T细胞系JM和CEM中观察到了原癌基因N-myc和lck的表达。L428细胞和CO细胞表达了原癌基因c-fes的异常转录本。因此，源自霍奇金病的细胞系在原癌基因的表达模式上是异质性的，表达了未转化造血细胞中未发现的原癌基因的正常和异常转录本。
