Dienerowitz Maria, Gibson Graham, Bowman Richard, Padgett Miles
SUPA, School of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ, UK.
Opt Express. 2011 Nov 21;19(24):24589-95. doi: 10.1364/OE.19.024589.
We investigate the effects of 1(st) order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1 μm polystyrene beads up to 50 μm deep in the sample.
我们研究了一阶球差和离焦对深入样品数十微米的光阱刚度的影响。我们使用空间光调制器来控制这两种像差。在一系列深度范围内保持最佳阱刚度的关键是离焦和轴向物镜位置的特定非平凡组合。这种优化将阱刚度提高了高达3倍，并允许在样品中捕获深度达50μm的1μm聚苯乙烯珠。
