Zhang Qing, Taguchi Ayumu, Schliekelman Mark, Wong Chee-Hong, Chin Alice, Kuick Rork, Misek David E, Hanash Samir
Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5-C800, Seattle, WA 98109, USA.
Int J Proteomics. 2011;2011:145010. doi: 10.1155/2011/145010. Epub 2011 Oct 29.
We have explored the potential of proteomic profiling to contribute to the delineation of the range of expression and subcellular localization of aldehyde dehydrogenases (ALDHs) in lung adenocarcinoma. In-depth quantitative proteomics was applied to 40 lung adenocarcinoma cell lines resulting in the identification of the known members of the ALDH family. Substantial heterogeneity in the level and occurrence of ALDHs in total lysates and on the cell surface and in their release into the culture media was observed based on mass spectrometry counts. A distinct pattern of expression of ALDHs was observed in cells exhibiting epithelial features relative to cells exhibiting mesenchymal features. Strikingly elevated levels of ALDH1A1 were observed in two cell lines. We also report on the occurrence of an immune response to ALDH1A1 in lung cancer.
我们探讨了蛋白质组学分析在描绘醛脱氢酶（ALDHs）在肺腺癌中的表达范围和亚细胞定位方面的潜力。对40个肺腺癌细胞系进行了深入的定量蛋白质组学分析，从而鉴定出了ALDH家族的已知成员。基于质谱计数，观察到总裂解物、细胞表面以及释放到培养基中的ALDHs水平和出现情况存在显著异质性。相对于表现出间充质特征的细胞，在表现出上皮特征的细胞中观察到了ALDHs的独特表达模式。在两个细胞系中观察到ALDH1A1水平显著升高。我们还报告了肺癌中对ALDH1A1的免疫反应情况。
