College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, People's Republic of China.
Enzyme Microb Technol. 2011 Jul 10;49(2):192-6. doi: 10.1016/j.enzmictec.2011.05.001. Epub 2011 May 13.
Research work was objectively targeted to synthesize highly pure diacylglycerol (DAG) with glycerolysis of soybean oil in a solvent medium of t-butanol. Three commercial immobilized lipases (Lipozyme RM IM, Lipozyme TL IM and Novozym 435) were screened, and Novozym 435 was the best out of three candidates. Batch reaction conditions of the enzymatic glycerolysis, the substrate mass ratio, the reaction temperature and the substrate concentration, were studied. The optimal reaction conditions were achieved as 6.23:1 mass ratio of soybean oil to glycerol, 40% (w/v) of substrate concentration in t-butanol and reaction temperature of 50 °C. A two-stage molecular distillation was employed for purification of DAG from reaction products. Scale-up was attempted based on the optimized reaction conditions, 98.7% (24 h) for the conversion rate of soybean oil, 48.5% of DAG in the glycerolysis products and 96.1% for the content of DAG in the final products were taken in account as the results.
研究工作的目标是客观地合成高纯度二酰基甘油（DAG），方法是在正丁醇溶剂介质中通过大豆油的甘油解反应。筛选了三种商业固定化脂肪酶（Lipozyme RM IM、Lipozyme TL IM 和 Novozym 435），其中 Novozym 435 是三种候选物中最好的一种。研究了酶促甘油解反应的分批反应条件、底物质量比、反应温度和底物浓度。最佳反应条件为：大豆油与甘油的质量比为 6.23:1，正丁醇中底物浓度为 40%（w/v），反应温度为 50°C。采用两级分子蒸馏从反应产物中纯化 DAG。根据优化的反应条件进行放大试验，24 小时的转化率为 98.7%，甘油解产物中 DAG 的含量为 48.5%，最终产物中 DAG 的含量为 96.1%。
