Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
Z Med Phys. 2012 Feb;22(1):40-7. doi: 10.1016/j.zemedi.2011.10.010. Epub 2011 Nov 22.
The aim of this study was to assess the applicability of autofluorescence (AF) spectroscopy as a method for the diagonosis of normal, benign and malignant of dysplasia in colorectal tissues experimentally. By improvement of optical design in laser pulse generator, wavelength-adjustable output was acquired and the optimal wavelength was defined as 380 nm. With 380-nm pulsed laser excitation, AF spectra of normal, benign and malignant colorectal tissues were recorded in the spectra region from 460-570 nm in vitro. The spectral analysis for discrimination among the different types of tissues was carried out using principal component analysis (PCA)-based Neural networks algorithm. The performance of analysis was pretty good with sensitivity, specificity and accuracy found to be 100%,90% and 96.7%, respectively. The AF spectroscopy may serve as an excellent tool for the evaluation of dysplasia in colorectal clinical diagnosis.
本研究旨在评估荧光光谱分析（AF）作为一种诊断方法在结直肠组织中的应用，该方法适用于诊断正常、良性和恶性病变以及异型增生。通过改进激光脉冲发生器的光学设计，获得了波长可调的输出，并将最佳波长定义为 380nm。采用 380nm 脉冲激光激发，在 460-570nm 光谱范围内记录了正常、良性和恶性结直肠组织的 AF 光谱。应用基于主成分分析（PCA）的神经网络算法对不同类型组织的光谱进行分析。分析结果性能良好，灵敏度、特异性和准确率分别为 100%、90%和 96.7%。AF 光谱分析可能成为结直肠临床诊断中评估异型增生的一种极好的工具。
