Berndtson W E, Thompson T L
Department of Animal and Nutritional Sciences, University of New Hampshire, Durham 03824.
J Androl. 1990 Jul-Aug;11(4):325-35.
Data from 25 rats aged 60, 150, or 240 days (n = 75) were used to determine the number of observations per rat and or rats per treatment group needed to provide future experiments of known power and sensitivity. End points examined included body weight, testicular weight, number of resistant elongated spermatids, yields of germ cells from their younger progenitors, and germ cell:Sertoli cell ratios. Requirements differed in relation to the experimental power and sensitivity desired. However, the replication needed for detecting equivalent treatment effects on paired testes weight or the number of spermatids per gram of testis increased with age, and was twice as great for 240- vs 60-day-old rats. In contrast, approximately twice as many 60-day-old rats were required for detecting treatment effects on the number of spermatids per Sertoli cell than when 150- or 240-day-old rats were used.
来自25只60日龄、150日龄或240日龄大鼠（n = 75）的数据用于确定每只大鼠的观察次数和/或每个治疗组所需的大鼠数量，以进行具有已知效力和敏感性的未来实验。检查的终点包括体重、睾丸重量、抗精子伸长细胞数量、其年轻祖细胞产生的生殖细胞产量以及生殖细胞与支持细胞的比例。所需的数量因期望的实验效力和敏感性而异。然而，检测对配对睾丸重量或每克睾丸精子细胞数量的等效治疗效果所需的重复次数随年龄增加，240日龄大鼠与60日龄大鼠相比所需次数是其两倍。相比之下，检测对每个支持细胞精子细胞数量的治疗效果时，与使用150日龄或240日龄大鼠相比，需要的60日龄大鼠数量大约是其两倍。
