Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
J Colloid Interface Sci. 2012 Feb 1;367(1):153-60. doi: 10.1016/j.jcis.2011.10.063. Epub 2011 Nov 6.
The effect of ionic strength of agarose solution and quenching temperature of the emulsion on the structure and mechanical strength of agarose-based chromatographic adsorbents was investigated. Solutions of agarose containing different amounts of NaCl were emulsified at elevated temperature in mineral oil using a high-shear mixer. The hot emulsion was quenched at different temperatures leading to the gelation of agarose and formation of soft particles. Analysis of Atomic Force Microscopy (AFM) images of particle surfaces shows that pore size of particles increases with ionic strength and/or high quenching temperature. Additionally it has been found that the compressive strength of particles measured by micromanipulation also increases with ionic strength of the emulsion and/or high quenching temperature but these two parameters have no significant effect on the resulting particle size and particle size distribution. Results from both characterization methods were compared with Sepharose 4B, a commercial agarose-based adsorbent. This is the first report examining the effect of ionic strength and cooling conditions on the microstructure of micron-sized agarose beads for bioseparation.
考察了琼脂糖溶液的离子强度和乳液的淬火温度对基于琼脂糖的色谱吸附剂的结构和机械强度的影响。在矿物油中使用高剪切混合器将含有不同量 NaCl 的琼脂糖溶液在高温下乳化。将热乳液在不同温度下淬火，导致琼脂糖凝胶化并形成软颗粒。颗粒表面原子力显微镜 (AFM) 图像分析表明，颗粒的孔径随离子强度和/或高淬火温度的增加而增大。此外，还发现通过微操作测量的颗粒压缩强度也随乳液的离子强度和/或高淬火温度的增加而增加，但这两个参数对所得颗粒尺寸和颗粒尺寸分布没有显著影响。两种表征方法的结果均与 Sepharose 4B（一种商业琼脂糖基吸附剂）进行了比较。这是首次研究离子强度和冷却条件对用于生物分离的微米级琼脂糖珠的微观结构的影响的报告。
