Serdiuk I N, Deriusheva E I
Biofizika. 2011 Sep-Oct;56(5):899-927.
The modern methods of research of biological molecules whose application led to the development of a new field of science, biophysics of single molecules, are reviewed. The measurement of the characteristics of single molecules enables one to reveal their individual features, and it is just for this reason that much more information can be obtained from one molecule than from the entire ensample of molecules. The high sensitivity of the methods considered in detail makes it possible to come close to the solution of the basic problem of practical importance, namely, the determination of the nucleotide sequence of a single DNA molecule.
本文综述了生物分子的现代研究方法，这些方法的应用推动了一个新的科学领域——单分子生物物理学的发展。对单分子特性的测量能够揭示其个体特征，正因为如此，从一个分子中获得的信息比从整个分子样本中获得的信息要多得多。详细讨论的这些方法具有高灵敏度，使得有可能接近解决具有实际重要性的基本问题，即确定单个DNA分子的核苷酸序列。
