Polonikov A V, Ushachev D V, Shestakov A M, Ivanov V P, Solodilova M A, Vialykh E K, Kozhukhov M A, Kolesnikova O E, Ivakin V E, Katargina L N, Kabanina V A, Kupriianova Ia S, Tevs D S
Kursk, Russia.
Kardiologiia. 2011;51(10):33-9.
In this study we for the first time in Russian population elucidated association between G460W polymorphism of -adducin gene (ADD1) and risk of development of hypertensive disease (HD). DNA samples from 205 patients with HD and 207 healthy nonrelated individuals of Russian nationality were genotyped for G460W polymorphism of ADD1 gene by polymerase chain reaction and restriction analysis. We detected no statistically significant differences between groups of healthy people and patients with HD. But among smokers with 460GW genotype of ADD1 gene we found elevated risk of HD development (OR 2.71, 95%CI 1.01-7.26; p=0.04). Among nonsmokers the given genotype did not influence risk of origination of the disease (OR 0.67, 95%CI 0.39-1.15; p=0.15). Moreover carriers of 460GW genotype who did not consume fresh vegetables and fruits or consumed them insufficiently (once a day or less) had the highest risk of HD development (OR 2.24, 95%CI 1.06-4.73; p=0.03) while in subjects who consumed fresh vegetables and fruits regularly the given genotype possessed protective properties in relation to risk of development of the disease (OR 0.25, 95%CI 0.09-0.68; p=0.005). Thus in the studied Russian population G460W polymorphism of ADD1 gene can be considered as predisposition gene to HD, but its pathological effect is manifested solely under influence of environmental factors.
在本研究中，我们首次在俄罗斯人群中阐明了β - 内收蛋白基因（ADD1）的G460W多态性与高血压疾病（HD）发生风险之间的关联。通过聚合酶链反应和限制性分析，对205例HD患者及207名俄罗斯籍健康非亲属个体的DNA样本进行了ADD1基因G460W多态性基因分型。我们发现健康人群组与HD患者组之间无统计学显著差异。但在携带ADD1基因460GW基因型的吸烟者中，我们发现HD发生风险升高（OR 2.71，95%CI 1.01 - 7.26；p = 0.04）。在非吸烟者中，该基因型不影响疾病发生风险（OR 0.67，95%CI 0.39 - 1.15；p = 0.15）。此外，未食用新鲜蔬菜和水果或食用不足（每天一次或更少）的460GW基因型携带者HD发生风险最高（OR 2.24，95%CI 1.06 - 4.73；p = 0.03），而在经常食用新鲜蔬菜和水果的受试者中，该基因型对疾病发生风险具有保护作用（OR 0.25，95%CI 0.09 - 0.68；p = 0.005）。因此，在本研究的俄罗斯人群中，ADD1基因的G460W多态性可被视为HD的易感基因，但其病理效应仅在环境因素影响下才会显现。
