Yan Dong, Jin Yishi
Howard Hughes Medical Institute, Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
Methods Cell Biol. 2011;106:413-43. doi: 10.1016/B978-0-12-544172-8.00015-3.
The complete known anatomical connections of C. elegans nervous system have provided researchers ample opportunities to discover fundamental principles underlying neuronal development. Transgenic labeling with fluorescent proteins in neuronal cells has had an unparalleled impact on our ability to visualize cellular architecture and dynamics. In this chapter, we summarize the common methods and guidelines for dissecting the molecular and cellular mechanisms controlling nervous system formation. We end with a brief description of several applications that illustrate the use of these methods.
秀丽隐杆线虫神经系统完整的已知解剖学连接为研究人员提供了充足的机会来发现神经元发育的基本原理。在神经元细胞中用荧光蛋白进行转基因标记对我们可视化细胞结构和动态的能力产生了无与伦比的影响。在本章中，我们总结了剖析控制神经系统形成的分子和细胞机制的常用方法及指南。最后，我们简要描述了几个应用实例，以说明这些方法的用途。
