Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
J Dig Dis. 2011 Dec;12(6):481-8. doi: 10.1111/j.1751-2980.2011.00521.x.
To ascertain serum and tissue expression of des-gamma-carboxyprothrombin (DCP) in patients with hepatocellular carcinoma (HCC) and liver cirrhosis and clarify the relationship between DCP expression and prognosis.
Expression of DCP in tissues was evaluated with immunohistochemical staining using anti-DCP antibody in 74 patients with a single primary HCC nodule and liver cirrhosis. Their serum DCP levels were determined using an enzyme immunoassay with a double antibody sandwich system.
Positive DCP expression in cancerous and non-cancerous tissues was related to a worse prognosis for patients with HCC and liver cirrhosis. The combined evaluation of tissue DCP expression and serum DCP level showed that prognosis was the worst for patients with positive tissue DCP expression and a high serum DCP level. Univariate analysis indicated that a lower 5-year survival rate was significantly correlated with positive tissue DCP expression, a high serum DCP level and the combined factor of positive tissue DCP expression and a high serum DCP level. Multivariate analysis indicated that the combined factor of positive tissue DCP expression and a high serum DCP level was a significant prognostic factor.
The combined evaluation of tissue DCP expression and serum DCP level is more useful than either factor alone in predicting prognosis for patients with HCC and liver cirrhosis.
确定血清和组织中去γ-羧基凝血酶原（DCP）在肝细胞癌（HCC）和肝硬化患者中的表达情况，并阐明 DCP 表达与预后之间的关系。
采用抗 DCP 抗体的免疫组织化学染色法检测 74 例单个原发性 HCC 结节和肝硬化患者组织中 DCP 的表达，采用双抗体夹心酶联免疫吸附法测定其血清 DCP 水平。
癌组织和非癌组织中 DCP 的阳性表达与 HCC 和肝硬化患者的预后较差有关。组织 DCP 表达和血清 DCP 水平的联合评估显示，组织 DCP 阳性表达和血清 DCP 水平高的患者预后最差。单因素分析表明，5 年生存率较低与组织 DCP 阳性表达、血清 DCP 水平升高以及组织 DCP 阳性表达和血清 DCP 水平升高的联合因素显著相关。多因素分析表明，组织 DCP 阳性表达和血清 DCP 水平升高的联合因素是一个显著的预后因素。
组织 DCP 表达和血清 DCP 水平的联合评估比单独评估任何一个因素更能预测 HCC 和肝硬化患者的预后。
