Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
Meat Sci. 2012 Mar;90(3):865-9. doi: 10.1016/j.meatsci.2011.11.008. Epub 2011 Nov 9.
The aim of this study was to evaluate the transfer of pathogens population to non-inoculated beef fillets through meat mincing machine. In this regard, cocktails of mixed strain cultures of each Listeria monocytogenes, Salmonella enterica ser. Typhimurium and Escherichia coli O157:H7 were used for the inoculation of beef fillets. Three different initial inoculum sizes (3, 5, or 7 log CFU/g) were tested. The inoculated beef fillets passed through meat mincing machine and then, six non-inoculated beef fillets passed in sequence through the same mincing machine without sanitation. The population of each pathogen was measured. It was evident that, all non-inoculated beef fillets were contaminated through mincing with all pathogens, regardless the inoculum levels used. This observation can be used to cover knowledge gaps in risk assessments since indicates the potential of pathogen contamination and provides significant insights for the risk estimation related to cross-contamination, aiming thus to food safety enhancement.
本研究旨在评估通过绞肉机将病原体种群转移至未接种牛肉薄片的情况。为此，使用混合菌株培养物的李斯特菌单增李斯特菌、肠炎沙门氏菌血清型 Typhimurium 和大肠杆菌 O157:H7 的混合物对牛肉薄片进行接种。测试了三个不同的初始接种物大小（3、5 或 7 log CFU/g）。接种的牛肉薄片通过绞肉机，然后，六个未接种的牛肉薄片连续通过同一台未进行消毒的绞肉机。测量每种病原体的数量。显然，无论使用的接种物水平如何，所有未接种的牛肉薄片都通过绞肉被所有病原体污染。这一观察结果可以用来填补风险评估中的知识空白，因为它表明了病原体污染的潜力，并为与交叉污染相关的风险估计提供了重要的见解，从而旨在提高食品安全。
