Hashizume K, DeGroot L J
Endocrinology. 1979 Jul;105(1):204-9. doi: 10.1210/endo-105-1-204.
A heat-stable protein which inhibits cAMP dependent protein kinase was prepared from the bovine thyroid 100,000 X g supernatant. This protein inhibited the cAMP-dependent protein kinase both from bovine thyroid cytosol and bovine thyroid plasma membranes. The inhibitory effect was noncompetitive with histone as substrate of the cytosolic enzyme. The stimulatory modulator for cGMP-dependent protein kinase was separated from the 100,000 X g supernatant by Sephadex G-100 gel filtration. The stimulatory modulator had no stimulating effect on cAMP-dependent phosphorylation, and the inhibitory modulator of cAMP-dependent enzyme had no effect on cGMP-requiring phosphorylations. The inhibitory modulator may regulate cAMP-dependent protein kinase activity in cytosol and plasma membrane, and the stimulatory modulator for cGMP-dependent protein kinase may have a role in thyroid function independent of the cAMP-requiring system.
一种抑制环磷酸腺苷（cAMP）依赖性蛋白激酶的热稳定蛋白是从牛甲状腺100,000×g上清液中制备的。该蛋白抑制来自牛甲状腺胞质溶胶和牛甲状腺质膜的cAMP依赖性蛋白激酶。对于作为胞质酶底物的组蛋白，其抑制作用是非竞争性的。通过葡聚糖G - 100凝胶过滤从100,000×g上清液中分离出环磷酸鸟苷（cGMP）依赖性蛋白激酶的刺激调节剂。该刺激调节剂对cAMP依赖性磷酸化没有刺激作用，而cAMP依赖性酶的抑制调节剂对需要cGMP的磷酸化没有影响。抑制调节剂可能调节胞质溶胶和质膜中的cAMP依赖性蛋白激酶活性，并且cGMP依赖性蛋白激酶的刺激调节剂可能在甲状腺功能中发挥作用，而与需要cAMP的系统无关。
