de Soet J J, Holbrook W P, van Amerongen W E, Schipper E, Homburg C H, de Graaff J
Department of Oral Microbiology, Academic Centre for Dentistry, Amsterdam (ACTA), The Netherlands.
ASDC J Dent Child. 1990 Sep-Oct;57(5):337-42.
The aim of this study was to investigate the presence of S. sobrinus and S. mutans in specimens of dental plaque and saliva of children five years of age in Reykjavik, Iceland (study 1) and in samples of dental plaque from children nine years of age in Amsterdam, The Netherlands (study 2). The immuneblotting technique (IBT) was a suitable method to evaluate the presence and numbers of S. mutans and S. sobrinus in human dental plaque and saliva. In study 1, eighty-four children were evaluated bacteriologically; of these, 73 percent harbored mutans streptococci in their plaque or saliva. S. sobrinus similarly was present in 29 percent of the children. In study 2 (seventy-two children), the corresponding percentages were 81 percent for S. mutans, and 35 percent for S. sobrinus. The latter was detected in 6 percent of the plaque samples exclusive of S. mutans.
本研究的目的是调查冰岛雷克雅未克5岁儿童牙菌斑和唾液样本（研究1）以及荷兰阿姆斯特丹9岁儿童牙菌斑样本（研究2）中嗜sobrinus链球菌和变形链球菌的存在情况。免疫印迹技术（IBT）是评估人牙菌斑和唾液中变形链球菌和嗜sobrinus链球菌的存在情况及数量的合适方法。在研究1中，对84名儿童进行了细菌学评估；其中，73%的儿童牙菌斑或唾液中携带变形链球菌。嗜sobrinus链球菌同样存在于29%的儿童中。在研究2（72名儿童）中，变形链球菌和嗜sobrinus链球菌的相应百分比分别为81%和35%。在不包括变形链球菌的6%的牙菌斑样本中检测到了后者。
