Thylstrup A, Boyar R M, Holmen L, Bowden G H
Department of Cariology and Endodontics, Royal Dental College, Copenhagen, Denmark.
J Dent Res. 1990 Oct;69(10):1626-33. doi: 10.1177/00220345900690100101.
Eleven children, each having one or two pairs of premolars to be extracted for orthodontic purposes, participated in the study. The model involved placement of a special orthodontic band that allowed the accumulation of plaque in a defined area between the band and the buccal enamel. Examination of enamel changes was carried out in experimental teeth that had been exposed to local plaque accumulation for one, two, four, eight or 14 days. The specimens were examined under the light (LM) and the scanning electron microscope (SEM). All teeth had signs of very mild dental fluorosis. No indications of demineralization were noted after one day. SEM examination showed signs of crystal dissolution in some of the two-day specimens. Six of eight four-day specimens exhibited surface dissolution. All eight- and 14-day specimens showed signs of surface demineralization in the LM as well as in the SEM. These observations documented that undisturbed bacterial deposits are capable of initiating enamel demineralization within short time periods, even in children living in a water-fluoridated area.
11名儿童参与了该研究，每名儿童都有一两对前磨牙需要为正畸目的而拔除。该模型包括放置一个特殊的正畸带环，该带环允许在带环与颊侧牙釉质之间的特定区域积聚牙菌斑。对暴露于局部牙菌斑积聚1天、2天、4天、8天或14天的实验牙齿进行牙釉质变化检查。在光学显微镜（LM）和扫描电子显微镜（SEM）下对标本进行检查。所有牙齿均有非常轻微的氟斑牙迹象。1天后未发现脱矿迹象。扫描电子显微镜检查显示，一些两天的标本有晶体溶解迹象。八个四天的标本中有六个表现出表面溶解。所有八天和十四天的标本在光学显微镜和扫描电子显微镜下均显示出表面脱矿迹象。这些观察结果证明，即使在生活在水氟化地区的儿童中，未受干扰的细菌沉积物也能够在短时间内引发牙釉质脱矿。
