光动力疗法和内皮抑素对裸鼠人胶质瘤异种移植物的影响。
Effect of photodynamic therapy and endostatin on human glioma xenografts in nude mice.
机构信息
Department of Neurosurgery, The Fourth Affiliated Hospital, Haerbin Medical University, Harbin 150001, China.
出版信息
Photodiagnosis Photodyn Ther. 2011 Dec;8(4):314-20. doi: 10.1016/j.pdpdt.2011.04.002. Epub 2011 May 19.
The aim of this study was to investigate the effect of endostar (a recombinant human endostatin) and photodynamic therapy (PDT) on gliomas. To establish glioma xenografts, human U251 glioma cells were injected into the brain of nude mice. Mice with MRI-confirmed glioma received hematoporphyrin monomethyl ether (HMME)-mediated PDT, daily injection of endostar or their combination, respectively. After treatment, tumor volume, the expression of HIF-1α, VEGF-A and apoptosis marker, and animal survival were examined. PDT and endostar treatment can prolong survival. Changes in induction of apoptosis, tumor growth and survival were more significant in PDT+endostar group. After PDT, HIF-1α and VEGF-A expressions were markedly increased. After endostar treatment, HIF-1α and VEGF-A expressions were significantly reduced. PDT in combination with endostar can significantly inhibit the growth of glioma xenografts. This approach may represent a promising strategy in the treatment of glioma.
本研究旨在探讨恩度（重组人血管内皮抑制素）联合光动力疗法（PDT）对脑胶质瘤的作用。将人 U251 脑胶质瘤细胞注射到裸鼠脑内，建立脑胶质瘤移植瘤模型。MRI 证实有脑胶质瘤的小鼠分别接受血卟啉单甲醚（HMME）介导的 PDT、每天注射恩度或两者联合治疗。治疗后，检测肿瘤体积、缺氧诱导因子-1α（HIF-1α）、血管内皮生长因子-A（VEGF-A）和凋亡标志物的表达以及动物的存活情况。PDT 和恩度治疗均可延长生存期。PDT+恩度组在诱导凋亡、肿瘤生长和生存方面的变化更为显著。PDT 后，HIF-1α 和 VEGF-A 的表达明显增加。恩度治疗后，HIF-1α 和 VEGF-A 的表达明显降低。PDT 联合恩度可显著抑制脑胶质瘤异种移植瘤的生长。这种方法可能是治疗脑胶质瘤的一种有前途的策略。
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