Zeng Zhi, Xie Runqian, Zhang Tao, Zhang Han, Chen Jie Yu
School of Chemistry and Environment, South China Normal University, Guangzhou, P.R. China.
J Oleo Sci. 2011;60(12):591-6. doi: 10.5650/jos.60.591.
The chemical compositions of volatile components from Magnolia biondii Pamp were determined by steam distillation (SD) and headspace solid phase micro-extraction (HS-SPME) followed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. Fifty-six compounds were identified and the major volatile components were d-camphor (0.18-43.26%), 1,8-cineol (13.23-38.02%), α-terpineol (6.57-12.29%) and α-cadinene (5.53-15.15%). The comparison of the volatile components from M. biondii Pamp harvested in three regions of China was investigated. Also, the comparison of volatile components by SD and HS-SPME methods in term of isolation time, plant-consuming and chemical compositions was discussed as well. The percentages of the volatile components by HS-SPME method were found to be large difference from the corresponding one by SD method. HS-SPME technique was much faster than SD (60 min (HS-SPME)/420 min (SD)). Although the aromatic profiles between HS-SPME and SD methods showed several quantitative differences, HS-SPME may be applied routinely to analyze aromatic and medicinal plants.
采用水蒸气蒸馏（SD）法和顶空固相微萃取（HS-SPME）法，结合气相色谱（GC）和气相色谱-质谱联用（GC-MS）分析技术，对望春玉兰挥发性成分的化学组成进行了测定。共鉴定出56种化合物，主要挥发性成分有d-樟脑（0.18 - 43.26%）、1,8-桉叶素（13.23 - 38.02%）、α-松油醇（6.57 - 12.29%）和α-杜松烯（5.53 - 15.15%）。对采自中国三个地区的望春玉兰挥发性成分进行了比较研究。此外，还从分离时间、植物消耗量和化学组成等方面，对望春玉兰挥发性成分的水蒸气蒸馏法和顶空固相微萃取法进行了比较。结果发现，顶空固相微萃取法所得挥发性成分的百分含量与水蒸气蒸馏法相应成分的百分含量有很大差异。顶空固相微萃取技术比水蒸气蒸馏法快得多（60分钟（顶空固相微萃取）/420分钟（水蒸气蒸馏））。尽管顶空固相微萃取法和水蒸气蒸馏法的香气图谱在定量方面存在一些差异，但顶空固相微萃取法可常规应用于芳香植物和药用植物的分析。
