青海-西藏高原草原优势种薹草属植物拟斯卑尔脱薹草的微卫星引物。
Microsatellite primers in Carex moorcroftii (Cyperaceae), a dominant species of the steppe on the Qinghai-Tibetan Plateau.
机构信息
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai, China.
出版信息
Am J Bot. 2011 Dec;98(12):e382-4. doi: 10.3732/ajb.1100105. Epub 2011 Nov 28.
PREMISE OF THE STUDY
Microsatellite markers were developed in Carex moorcroftii, a rhizomatous clonal sedge that dominates the steppes and meadows of the Qinghai-Tibetan Plateau.
METHODS AND RESULTS
Using the combined biotin capture method, 30 microsatellite primer sets were isolated and characterized. Twenty-two of these markers showed polymorphism, and the number of alleles per locus ranged from two to 11 across 96 individuals from four populations.
CONCLUSIONS
These markers provide a useful tool to investigate the clonal structure and mating system of C. moorcroftii.
研究前提
微卫星标记在毛果苔草中开发，毛果苔草是一种根茎状克隆莎草，主导着青藏高原的草原和草地。
方法和结果
使用联合生物素捕获法，分离并鉴定了 30 个微卫星引物。在来自四个群体的 96 个个体中，有 22 个标记显示多态性，每个位点的等位基因数从两个到 11 个不等。
结论
这些标记为研究毛果苔草的克隆结构和交配系统提供了有用的工具。
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