Sarlo K, Clark E D, Ryan C A, Bernstein D I
Procter & Gamble Co., Miami Valley Laboratories, Cincinnati, Ohio 45239-8707.
J Allergy Clin Immunol. 1990 Sep;86(3 Pt 1):393-9. doi: 10.1016/s0091-6749(05)80103-4.
An ELISA was developed to detect specific IgE antibody to the Bacillus subtilis-derived proteolytic detergent enzyme, subtilisin A (Alcalase), in sera from exposed detergent workers. Workers in the detergent industry are exposed via inhalation to low levels of the enzyme dust in the presence of detergent dust. Chemically inactivated Alcalase was used as the test antigen. Significant binding of IgE antibody to the immobilized enzyme was detected in the ELISA. The binding of allergic antibody to Alcalase was specifically inhibited in a dose-dependent manner by preincubating sera with 0.1 to 100 micrograms of inactivated Alcalase. Binding of IgE antibody to Alcalase could not be inhibited by two other inactivated bacterial proteases, Savinase and Esperase, derived from different Bacillus species. ELISA and skin test results demonstrated total agreement for 27 of 31 samples (87%), whereas RAST and skin test results demonstrated total agreement for only 24 of 31 samples (77%), indicating that the ELISA was more sensitive than the RAST. We conclude that the ELISA is a sensitive, fast, alternative to the RAST for detection of Alcalase-specific IgE antibody in detergent enzyme-exposed workers.
开发了一种酶联免疫吸附测定法（ELISA），用于检测接触洗涤剂的工人血清中针对枯草芽孢杆菌衍生的蛋白水解洗涤剂酶枯草杆菌蛋白酶A（碱性蛋白酶）的特异性IgE抗体。洗涤剂行业的工人在洗涤剂粉尘存在的情况下，通过吸入接触低水平的酶粉尘。化学灭活的碱性蛋白酶用作检测抗原。在ELISA中检测到IgE抗体与固定化酶的显著结合。通过将血清与0.1至100微克灭活的碱性蛋白酶预孵育，过敏抗体与碱性蛋白酶的结合以剂量依赖的方式被特异性抑制。IgE抗体与碱性蛋白酶的结合不能被另外两种源自不同芽孢杆菌属的灭活细菌蛋白酶，即沙维诺酶和埃斯帕诺酶所抑制。ELISA和皮肤试验结果显示，31个样本中有27个（87%）完全一致，而放射性变应原吸附试验（RAST）和皮肤试验结果显示，31个样本中只有24个（77%）完全一致，这表明ELISA比RAST更敏感。我们得出结论，ELISA是一种灵敏、快速的方法，可替代RAST用于检测接触洗涤剂酶的工人中碱性蛋白酶特异性IgE抗体。
