Unité MEDyC, UMR URCA CNRS 6237, IFR53, Faculté de Pharmacie, Université de Reims, Reims, France.
Epigenomics. 2011 Dec;3(6):785-94. doi: 10.2217/epi.11.102.
Classical biochemical and molecular methods for discerning cells with epigenetic modifications are often biologically perturbing or even destructive. We wondered whether the noninvasive laser tweezer Raman spectroscopy technique allowed the discrimination of single living human cells undergoing epigenetic modifications.
MATERIALS & METHODS: Human Jurkat leukemic cells were treated with inhibitors of histone deacetylases (trichostatin A and MS-275). Epigenetic changes were monitored through histone electrophoresis, nuclear image cytometry and laser tweezer Raman spectroscopy.
Treatment of Jurkat cells with histone deacetylase inhibitors increased histone acetylation and induced chromatin organization changes. Characteristic vibrations, issued from laser tweezer Raman spectroscopy analyses, mostly assigned to DNA and proteins allowed discerning histone deacetylase inhibitor-treated cells from control with high confidence. Statistical processing of laser tweezer Raman spectroscopy data led to the definition of specific biomolecular fingerprints of each cell group.
This original study shows that laser tweezer Raman spectroscopy is a label-free rapid tool to identify living cells that underwent epigenetic changes.
用于识别发生表观遗传修饰的细胞的经典生化和分子方法通常具有生物干扰性，甚至具有破坏性。我们想知道非侵入性的激光镊子拉曼光谱技术是否能够区分发生表观遗传修饰的单个活人体细胞。
用组蛋白去乙酰化酶抑制剂（曲古抑菌素 A 和 MS-275）处理人 Jurkat 白血病细胞。通过组蛋白电泳、核图像细胞术和激光镊子拉曼光谱监测表观遗传变化。
用组蛋白去乙酰化酶抑制剂处理 Jurkat 细胞会增加组蛋白乙酰化，并诱导染色质结构变化。从激光镊子拉曼光谱分析中发出的特征振动，主要归因于 DNA 和蛋白质，可高度确信地区分经组蛋白去乙酰化酶抑制剂处理的细胞与对照细胞。对激光镊子拉曼光谱数据的统计处理导致定义了每个细胞群的特定生物分子指纹。
这项原始研究表明，激光镊子拉曼光谱是一种无需标记的快速工具，可用于识别发生表观遗传变化的活体细胞。
