Institute of Clinical Chemistry, Hospital of the University of Munich, Munich, Germany.
Clin Chem Lab Med. 2011 Nov 30;50(3):521-4. doi: 10.1515/CCLM.2011.802.
Extensive sets of data are required to investigate the potential use of a therapeutic drug monitoring with individualization of dosage of the antimycotic compound caspofungin. The goal was to develop an improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for this aim.
Following protein precipitation, on-line solid phase extraction was performed for sample preparation. As the internal standard compound the veterinary drug tylosin was used. A standard validation protocol was applied.
Good reproducibility and accuracy of the method were observed. On-line solid phase extraction resulted in a convenient work-flow and good robustness of the method.
This improved LC-MS/MS method was found reliable and convenient. It can be suggested for further work on the clinical pharmacology of caspofungin in the setting of clinical research laboratories.
为了研究抗真菌药物卡泊芬净剂量个体化的治疗药物监测的潜在用途，需要大量的数据。目的是为此目的开发一种改进的液相色谱-串联质谱（LC-MS/MS）方法。
采用蛋白沉淀法进行样品前处理，在线固相萃取。以兽用药物泰乐菌素作为内标化合物。应用标准验证方案。
该方法具有良好的重现性和准确性。在线固相萃取使该方法具有方便的工作流程和良好的稳健性。
该改进的 LC-MS/MS 方法可靠且方便。它可以在临床研究实验室中用于进一步研究卡泊芬净的临床药理学。
