Kripke M L, Munn C G, Jeevan A, Tang J M, Bucana C
Department of Immunology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
J Immunol. 1990 Nov 1;145(9):2833-8.
These studies address the hypothesis that Ag-bearing epidermal Langerhans cells migrate to the regional lymph node during contact sensitization and function as APC. Skin from C3H mice was grafted onto BALB/c nude mice, and 7 or 14 days later, the recipients were sensitized with FITC through the grafts. APC from lymph nodes draining the site of sensitization were capable of sensitizing C3H recipients to FITC. Because sensitization is MHC restricted, only cells reaching the lymph node from the grafted skin could have induced contact hypersensitivity in C3H mice. Examination of the FITC+ draining lymph node cells by immunofluorescence and immunoelectron microscopy demonstrated that all were Ia+, most were F4/80+, and some contained Birbeck granules. These studies demonstrate that Ia+, FITC+ cells from the skin, at least some of which are Langerhans cells, leave the skin after epicutaneous sensitization with FITC and participate in the initiation of the contact hypersensitivity response within the regional lymph node.
在接触致敏过程中，携带抗原的表皮朗格汉斯细胞迁移至局部淋巴结并作为抗原呈递细胞发挥作用。将C3H小鼠的皮肤移植到BALB/c裸鼠身上，7或14天后，通过移植皮肤用异硫氰酸荧光素（FITC）对受体进行致敏。来自致敏部位引流淋巴结的抗原呈递细胞能够使C3H受体对FITC致敏。由于致敏受主要组织相容性复合体（MHC）限制，只有从移植皮肤到达淋巴结的细胞才能在C3H小鼠中诱导接触性超敏反应。通过免疫荧光和免疫电子显微镜检查FITC+引流淋巴结细胞发现，所有细胞均为Ia+，大多数为F4/80+，有些含有伯贝克颗粒。这些研究表明，皮肤来源的Ia+、FITC+细胞，其中至少一些是朗格汉斯细胞，在用FITC经皮致敏后离开皮肤，并参与局部淋巴结内接触性超敏反应的启动。
