Su S D, Ward M M, Apicella M A, Ward R E
Department of Molecular Immunology, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Immunol. 1990 Nov 1;145(9):2994-3001.
LPS is the major surface glycolipid on gram-negative bacteria. In this work, we have idiotypically characterized the antibody response against LPS in different species. To do this, we have produced mAb against LPS. Binding of many of these antibodies to LPS could be inhibited by LPS and lipid A, indicating that the monoclonals are specific for lipid A, the toxic moiety of the LPS molecule. One anti-lipid A antibody, IC9, proved protective against gram-negative bacteremia and endotoxic shock in murine protection models. We generated anti-idiotypic antibodies against IC9. The binding of several of these anti-Id to IC9 was specifically inhibited by lipid A. We used these anti-Id to characterize the anti-LPS response, and the results revealed that the IC9 Id is conserved in different species. The importance of an interspecies cross-reactive Id in the response to endotoxin and its relevance in vaccine development for septic shock are discussed.
脂多糖（LPS）是革兰氏阴性菌主要的表面糖脂。在本研究中，我们以独特型特征描述了不同物种针对LPS的抗体反应。为此，我们制备了抗LPS的单克隆抗体（mAb）。许多此类抗体与LPS的结合可被LPS和脂质A抑制，这表明这些单克隆抗体对脂质A具有特异性，脂质A是LPS分子的毒性部分。在小鼠保护模型中，一种抗脂质A抗体IC9被证明对革兰氏阴性菌血症和内毒素休克具有保护作用。我们制备了针对IC9的抗独特型抗体。脂质A可特异性抑制其中几种抗独特型抗体与IC9的结合。我们利用这些抗独特型抗体来表征抗LPS反应，结果显示IC9的独特型在不同物种中是保守的。本文讨论了种间交叉反应性独特型在内毒素反应中的重要性及其在脓毒性休克疫苗研发中的相关性。
