Dammeyer Thorben
Physical & Theoretical Chemistry, NanoBioSciences, TU-Braunschweig and Helmholtz Centre for Infection Research, Braunschweig, Germany.
Bioeng Bugs. 2012 Jan 1;3(1):67-71. doi: 10.4161/bbug.3.1.17739.
Recombinant antibody fragments have a wide range of applications from research to diagnostics and therapy. Of special interest are small fragments like fragment antigen binding (Fab) or single chain fragment variables (scFv) fragments as they can be produced inexpensively in bacterial expression systems. However, recombinant production efficiencies from established production hosts vary significantly leading to inadequate yields. Gene sequences that have been synthetically adapted to match the codon preferences and respective genomic tRNA pool of the host have been used to improve yields but cannot resolve the principal problem. The development of inducible broad host range scFv expression plasmid constructs leads the way to an easy and efficient screening method for the identification of the optimal bacterial expression host.
重组抗体片段在从研究到诊断及治疗等广泛领域都有应用。特别令人感兴趣的是小片段，如抗原结合片段（Fab）或单链可变片段（scFv），因为它们可以在细菌表达系统中低成本生产。然而，既定生产宿主的重组生产效率差异很大，导致产量不足。已使用经过合成调整以匹配宿主密码子偏好和相应基因组tRNA库的基因序列来提高产量，但无法解决主要问题。可诱导的广泛宿主范围scFv表达质粒构建体的开发为鉴定最佳细菌表达宿主提供了一种简便高效的筛选方法。
