大鼠中性粒细胞对诱变基因（Nrm-1）的反应与刺鼠基因座的连锁关系。

Linkage of neutrophil response to a mutagen gene (Nrm-1) to the agouti locus in the rat.

作者信息

Stolc V

机构信息

Department of Pathology, School of Medicine, University of Pittsburgh, PA 15261.

出版信息

J Immunogenet. 1990 Feb-Apr;17(1-2):1-8. doi: 10.1111/j.1744-313x.1990.tb00854.x.

DOI:10.1111/j.1744-313x.1990.tb00854.x

PMID:2212696

Abstract

The ACI or BDIV rats responded with decreased or increased neutrophil levels in blood after the N-methyl-N-nitrosourea (MNU) administration. The F1 hybrids had decreased neutrophil counts, and the BDIV x (BDIV x ACI)F1 backcross offspring showed two phenotypes. The sex of the rats and the neutrophil response to MNU assorted independently. The results indicated that the neutrophil response to MNU was regulated by autosomal gene Nrm-1 with two alleles. The Nrm-1d regulates the decrease and the Nrm-1i regulates the increase of neutrophils in blood after the MNU administration. The results were confirmed by the SKUMIX computer program. We found that the Nrm-1 gene was linked to the agouti locus (chi-square = 10.3, P less than 0.001). The map distance between two genes was 33 +/- 5 cM. The Nrm-1 gene thus resides on the linkage group IV of the rat.

摘要

给予N-甲基-N-亚硝基脲（MNU）后，ACI或BDIV大鼠血液中的中性粒细胞水平出现降低或升高。F1杂种的中性粒细胞计数减少，BDIV x (BDIV x ACI)F1回交后代表现出两种表型。大鼠的性别与对MNU的中性粒细胞反应独立分类。结果表明，对MNU的中性粒细胞反应由具有两个等位基因的常染色体基因Nrm-1调控。Nrm-1d调控MNU给药后血液中中性粒细胞的减少，Nrm-1i调控其增加。结果通过SKUMIX计算机程序得到证实。我们发现Nrm-1基因与刺鼠基因座连锁（卡方=10.3，P小于0.001）。两个基因之间的图距为33 +/- 5 cM。因此，Nrm-1基因位于大鼠的连锁群IV上。