Faculdade de Biociências e Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre/RS, Brazil.
Dev Psychobiol. 2012 Nov;54(7):706-13. doi: 10.1002/dev.20620. Epub 2011 Nov 29.
The aim of this study was to test the hypothesis that the renin-angiotensin system (RAS) components, as well as the oxidative stress system, would respond to early environmental changes. Thus, we have evaluated the effects of neonatal handling on both brain and kidney RAS and oxidative stress. Pups were divided into two groups: nonhandled and handled. The procedure consisted of handling them for 1 min/day in the first 10 days of life. On days 1, 5, and 10, animals were killed by decapitation. Blood samples were collected and the brain and kidneys were removed. Renin, AT(1), and AT(2) mRNA expression were evaluated through RT-PCR. Angiotensin II (ANG II) serum concentration was also measured. An increased ANG II concentration, brain and kidney AT(2) mRNA expression were demonstrated. The kidney mRNA AT(1) expression was decreased. There was also a kidney lipid peroxidation increase and a brain superoxide dismutase and catalase decrease. In conclusion, handling in the neonatal period induces the activation of the angiotensinergic system, as well as modulates its mRNA receptor expression. The oxidative stress balance system seems not to be involved.
本研究旨在验证以下假设，即肾素-血管紧张素系统（RAS）成分以及氧化应激系统会对早期环境变化作出反应。因此，我们评估了新生期处理对大脑和肾脏 RAS 及氧化应激的影响。将幼仔分为两组：未处理组和处理组。该过程包括在生命的前 10 天每天处理 1 分钟。在第 1、5 和 10 天，通过断头处死动物。收集血液样本并取出大脑和肾脏。通过 RT-PCR 评估肾素、AT(1)和 AT(2)mRNA 的表达。还测量了血管紧张素 II (ANG II) 的血清浓度。结果显示，ANG II 浓度升高，大脑和肾脏 AT(2)mRNA 表达增加，肾脏 AT(1)mRNA 表达减少。此外，肾脏脂质过氧化增加，大脑中超氧化物歧化酶和过氧化氢酶减少。总之，新生期处理会诱导血管紧张素能系统的激活，并调节其 mRNA 受体表达。氧化应激平衡系统似乎未参与其中。
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