INDIC ASAT AIP, Centro de Neurociencias, Panamá
Neuropsychiatr Dis Treat. 2011;7:697-706. doi: 10.2147/NDT.S25888. Epub 2011 Nov 17.
Studies have shown that Ras homolog enriched in striatum (Rhes) proteins are highly expressed in areas of the central nervous system that have high dopaminergic innervation. In this study, we used Rhes mutant mice (Wild type, Rhes KO, Rhes Heterozygous) of both sexes to explore differences in the effects of Rhes protein levels in basal levels of activity, anxiety, and stereotypy, in relation to sex. Adult male and female mice were evaluated in an open field test for measuring basal levels of activity and anxiety for 5 consecutive days, and they were tested in the apomorphine-induced stereotypy paradigm. Rhes protein levels affected basal levels of activity but it was not found to be related to sex differences. Moreover, a decrease in Rhes protein levels was linked to a nonsignificant anxiolytic effect, mainly in female mice. Finally, a decrease in Rhes protein levels does not affect dopamine D(1) and D(2) receptor (D(1)/D(2)) synergism in female or male mice. Together, these results suggest that Rhes protein levels affect locomotion activity, and have an influence in anxiety depending on sex; Rhes protein levels do not affect D(1)/D(2) synergism in both sexes.
研究表明，富含纹状体的 Ras 同源物（Rhes）蛋白在中枢神经系统中多巴胺能神经支配较高的区域高度表达。在这项研究中，我们使用了 Rhes 突变小鼠（野生型、Rhes KO、Rhes 杂合型）的雌雄两性，以探讨 Rhes 蛋白水平的差异对活动、焦虑和刻板行为的基础水平的影响，以及与性别相关的影响。成年雄性和雌性小鼠在开放场测试中连续 5 天进行了基础活动和焦虑水平的评估，并在阿扑吗啡诱导的刻板行为范式中进行了测试。Rhes 蛋白水平影响基础活动水平，但与性别差异无关。此外，Rhes 蛋白水平的降低与非显著的抗焦虑作用有关，主要在雌性小鼠中。最后，Rhes 蛋白水平的降低不会影响雌性或雄性小鼠多巴胺 D1 和 D2 受体（D1/D2）协同作用。总之，这些结果表明，Rhes 蛋白水平影响运动活动，并根据性别对焦虑产生影响；Rhes 蛋白水平在两性中均不影响 D1/D2 协同作用。
