用促性腺激素预处理对卵泡cAMP及对促性腺激素的类固醇反应的抑制作用

Suppression of follicular cAMP and steroid response to gonadotrophins by pretreatment with gonadotrophin.

作者信息

Weiss T J, Armstrong D T

出版信息

Mol Cell Endocrinol. 1979 Mar;13(3):229-39. doi: 10.1016/0303-7207(79)90083-2.

DOI:10.1016/0303-7207(79)90083-2

Abstract

This study examines the effect of preincubation of sheep ovarian follicles with hCG or FSH on the ability of isolated theca and granulosa to increase cAMP levels in response to a second incubation with hCG or FSH. Secondly, the effect of preincubation of small follicles with hCG on androstenedione and testosterone production is examined in intact follicles. Preincubation with hCG resulted in a decrease in the ability of theca from large (4--6 mm in diameter) and small (1--3 mm) follicles and granulosa from large follicles to increase cAMP levels in response to a second exposure to hCG. Similarly, preincubation with FSH suppressed the response of isolated granulosa from small and large follicles to a second exposure to FSH. A partial, but significant inhibition of the response to the other hormone was observed after preincubation with both FSH and hCG. Intact small follicles pretreated with hCG were unable to increase testosterone secretion in response to a second exposure to hCG.

摘要

本研究检测了用hCG或FSH对绵羊卵泡进行预孵育，对分离的卵泡膜细胞和颗粒细胞在再次用hCG或FSH孵育时增加cAMP水平能力的影响。其次，检测了用hCG对小卵泡进行预孵育对完整卵泡中雄烯二酮和睾酮生成的影响。用hCG预孵育导致大卵泡（直径4 - 6毫米）和小卵泡（直径1 - 3毫米）的卵泡膜细胞以及大卵泡颗粒细胞在再次暴露于hCG时增加cAMP水平的能力下降。同样，用FSH预孵育抑制了分离的小卵泡和大卵泡颗粒细胞对再次暴露于FSH的反应。在用FSH和hCG两者预孵育后，观察到对另一种激素反应的部分但显著抑制。用hCG预处理的完整小卵泡在再次暴露于hCG时无法增加睾酮分泌。