长期给予吗啡可改变大鼠心肌的蛋白质表达。
Prolonged morphine administration alters protein expression in the rat myocardium.
机构信息
Department of Physiology, Charles University, Prague.
出版信息
J Biomed Sci. 2011 Nov 30;18(1):89. doi: 10.1186/1423-0127-18-89.
BACKGROUND
Morphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alterations in rat myocardial protein levels after prolonged morphine treatment.
METHODS
Morphine was administered to adult male Wistar rats in high doses (10 mg/kg per day) for 10 days. Proteins from the plasma membrane- and mitochondria-enriched fractions or cytosolic proteins isolated from left ventricles were run on 2D gel electrophoresis, scanned and quantified with specific software to reveal differentially expressed proteins.
RESULTS
Nine proteins were found to show markedly altered expression levels in samples from morphine-treaded rats and these proteins were identified by mass spectrometric analysis. They belong to different cell pathways including signaling, cytoprotective, and structural elements.
CONCLUSIONS
The present identification of several important myocardial proteins altered by prolonged morphine treatment points to global effects of this drug on heart tissue. These findings represent an initial step toward a more complex view on the action of morphine on the heart.
背景
吗啡在临床上被用作一种高效的止痛药，也是治疗某些心脏病的首选药物。然而，关于其对心脏蛋白质表达的影响的信息却很缺乏。因此，在这里我们旨在确定长期吗啡治疗后大鼠心肌蛋白质水平的假定变化。
方法
将吗啡以高剂量（每天 10mg/kg）施用于成年雄性 Wistar 大鼠 10 天。从左心室分离的质膜和线粒体丰富部分或胞质蛋白的蛋白质在 2D 凝胶电泳上运行，用特定的软件进行扫描和定量，以揭示差异表达的蛋白质。
结果
在吗啡处理的大鼠样本中发现 9 种蛋白质的表达水平明显改变，通过质谱分析鉴定了这些蛋白质。它们属于不同的细胞途径，包括信号、细胞保护和结构元件。
结论
本研究首次确定了长期吗啡治疗后几种重要的心肌蛋白质的改变，这表明这种药物对心脏组织有全局影响。这些发现代表了对吗啡对心脏作用的更复杂观点的初步步骤。
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