Miyauchi M, Maruyama K
Department of Pathology, Chiba Cancer Center Research Institute, Japan.
J Electron Microsc (Tokyo). 1990;39(3):145-54.
HTLV-I particles grown in 4 human lymphocyte cultures were observed by electron microscopy. Particles in all cell lines showed marked size and structural variations that were characteristic of each cell line. Profiles of size-distributions were found to be similar among virus particles of the same origin in different cell lines. Immunogold electron microscopy using monoclonal antibody to HTLV-I-p15, -p19, or -p24 revealed varying reactivities of individual particles. By double-labeling technique using antibody to HTLV-I-p19 or -p24, particles were found to be classifiable into 4 groups: p19+p24+, p19+p24-, p19-p24%, and p19-p24-. Each cell line showed the characteristic profile of distributions of virus particles of each group as observed at different in vitro passage levels. These antibodies reacted with certain structures in the extracellular space and in the cytoplasm. Results of this study demonstrate variations in morphology and antigenicity, as well as the morphogenesis of HTLV-I particles in different host cells.
通过电子显微镜观察在4种人淋巴细胞培养物中生长的HTLV-I颗粒。所有细胞系中的颗粒均显示出明显的大小和结构变化，这些变化是每个细胞系所特有的。发现不同细胞系中相同来源的病毒颗粒之间的大小分布轮廓相似。使用针对HTLV-I-p15、-p19或-p24的单克隆抗体进行免疫金电子显微镜检查，揭示了单个颗粒的不同反应性。通过使用针对HTLV-I-p19或-p24的抗体的双标记技术，发现颗粒可分为4组：p19+p24+、p19+p24-、p19-p24%和p19-p24-。在不同的体外传代水平观察到，每个细胞系都显示出每组病毒颗粒分布的特征性轮廓。这些抗体与细胞外空间和细胞质中的某些结构发生反应。本研究结果证明了HTLV-I颗粒在不同宿主细胞中的形态和抗原性变化以及形态发生。
