Nutrition et Endocrinologie, LUNAM Université, Oniris, Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique, Atlanpôle-La Chantrerie, Nantes, France.
J Anim Physiol Anim Nutr (Berl). 2012 Oct;96(5):850-9. doi: 10.1111/j.1439-0396.2011.01263.x. Epub 2011 Nov 30.
Leptin is an adipocytokine mainly expressed by adipose tissue. Secretion of leptin in healthy animals is closely related to fat mass and metabolic activity. The aim of this study was to investigate plasma leptin variations, in relation to nutritional and exercise parameters in adult show horses during a work season. EDTA-blood samples were taken at rest from 37 Iberian horses. Body weight, body condition score and fat percentage determined by ultrasonic measurement of rump fat thickness were measured. Plasma leptin was determined with a multi-species RIA kit. Linear mixed effects model was used to assess relationship between plasma leptin and other biological parameters. Plasma leptin concentration was <12.6 ng/ml (mean = 2.8 ± 1.6 ng/ml) and was significantly higher during training periods (p < 0.0001) (4.5 ± 1.7 ng/ml) than in show periods (2.0 ± 1.1 ng/ml), despite a significant increase (p < 0.0001) in energy intake. The body weight remained almost constant. The plasma leptin concentrations were significantly affected by exercise (p < 0.0001), body weight (p = 0.04) and BCS (p < 0.0001), but were not affected by percentage of fat. In conclusion, the marked decrease in leptin values observed during a period of intense (i.e. excessive) exercise could result from an adaptation to cumulative alterations in energy balance, to exercise per se or to a combination of both.
瘦素是一种主要由脂肪组织表达的脂肪细胞因子。健康动物的瘦素分泌与脂肪量和代谢活性密切相关。本研究旨在探讨成年展示马在工作季节期间，与营养和运动参数相关的血浆瘦素变化。从 37 匹伊比利亚马中采集休息时的 EDTA 血液样本。通过测量臀部脂肪厚度的超声测量来确定体重、体况评分和脂肪百分比。使用多物种 RIA 试剂盒测定血浆瘦素。线性混合效应模型用于评估血浆瘦素与其他生物学参数之间的关系。血浆瘦素浓度<12.6ng/ml（平均值=2.8±1.6ng/ml），在训练期（p<0.0001）（4.5±1.7ng/ml）明显高于表演期（2.0±1.1ng/ml），尽管能量摄入显著增加（p<0.0001）。体重几乎保持不变。血浆瘦素浓度受运动（p<0.0001）、体重（p=0.04）和体况评分（p<0.0001）的显著影响，但不受脂肪百分比的影响。总之，在剧烈（即过度）运动期间观察到的瘦素值明显下降可能是由于对能量平衡的累积变化、运动本身或两者的组合的适应。
