Jeremiah Zaccheaus Awortu, Atiegoba Anne Ifeanyi, Mgbere Osaro
Haematology and Blood Transfusion Science Unit, Department of Medical Laboratory Sciences College of Health Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.
Hum Antibodies. 2011;20(3-4):71-5. doi: 10.3233/HAB-2011-0241.
The prevalence of antibodies to human platelet antigens (HPA) and human leukocyte antigens (HLA) class 1 antigens among Nigerian pregnant women has not been reported in our country. This study was therefore aimed at screening the obstetric population for evidence of alloimmunization due to human platelet and HLA class 1 antigens. One hundred and forty four (144) pregnant women attending the obstetric clinic of Military Hospital, Port Harcourt, participated in the study. Their sera were tested for antibodies to HPA and HLA class 1 antigens using GTI PakPlus solid phase ELISA Kit. The total prevalence rate of antibody production was 60.5% (87 out of 144). Among the positive samples, 60 had platelet glycoprotein specific antibodies (41.7%) and 27 had HLA class 1 antibodies (18.8%). In 39.6% of the pregnant women, both platelet specific antibodies and HLA class 1 antibodies appeared. The prevalence of platelet specific glycoprotein antibodies were obtained as follows: GP 11b/111a 12 (8.3%), GP 1a/11a 35 (20.8%), GP Ib/IX 18 (12.5%) and GP IV 9 (6.3%). The prevalence of each platelet antibody subgroup was obtained as follows: anti-HPA-1a,-3a,-4a (4.2%), anti-HPA-1b,-3b,-4a (4.2%), anti-HPA-30 5a and anti-GP Ib/IX (12.5% each), anti-HPA-5b (8.3%) and anti-GP IV (6.3%). A high prevalence rate of human platelet arid cytotoxic antibodies has been observed in our obstetric population. There is need to establish platelet serology laboratory for the proper antenatal and postnatal management of pregnant mothers in this region.
我国尚未报道过尼日利亚孕妇中人类血小板抗原（HPA）和人类白细胞抗原（HLA）-Ⅰ类抗原抗体的流行情况。因此，本研究旨在筛查产科人群中因人类血小板和HLA-Ⅰ类抗原导致的同种免疫证据。144名在哈科特港军事医院产科门诊就诊的孕妇参与了本研究。使用GTI PakPlus固相ELISA试剂盒检测她们血清中的HPA和HLA-Ⅰ类抗原抗体。抗体产生的总流行率为60.5%（144例中有87例）。在阳性样本中，60例有血小板糖蛋白特异性抗体（41.7%），27例有HLA-Ⅰ类抗体（18.8%）。39.6%的孕妇同时出现了血小板特异性抗体和HLA-Ⅰ类抗体。血小板特异性糖蛋白抗体的流行情况如下：GPⅡb/Ⅲa 12例（8.3%），GPⅠa/Ⅱa 35例（20.8%），GPⅠb/Ⅸ 18例（12.5%），GPⅣ 9例（6.3%）。各血小板抗体亚组的流行情况如下：抗-HPA-1a、-3a、-4a（各4.2%），抗-HPA-1b、-3b、-4a（各4.2%），抗-HPA-3、5a和抗-GPⅠb/Ⅸ（各12.5%），抗-HPA-5b（8.3%），抗-GPⅣ（6.3%）。在我们的产科人群中观察到人类血小板和细胞毒性抗体的高流行率。有必要建立血小板血清学实验室，以便对该地区的孕妇进行适当的产前和产后管理。
