疱疹病毒猿猴B病毒转化的淋巴样细胞系中的游离病毒DNA。

Episomal viral DNA in herpesvirus saimiri-transformed lymphoid cell lines.

作者信息

Werner F J, Bornkamm G W, Fleckenstein B, Mulder C

出版信息

IARC Sci Publ (1971). 1978(24 Pt 1):125-30.

Abstract

Structural analysis of episomal viral genomes from two herpesvirus saimiri (HVS)-transformed tumour cell lines (No. 1670 and 70N2) showed that both types of episomes have a higher molecular weight than linear virion DNA. The arrangement of unique (L) and repetitive (H) DNA in No. 1670 episomes was studied by partial denaturation mapping. Part of the L-sequences present in linear virion DNA was found to be missing, part was found to be duplicated in the episomes. The episomal L-DNA regions were correlated with the known physical gene maps of linear HVS DNA.

摘要

对来自两种赛氏疱疹病毒（HVS）转化的肿瘤细胞系（1670号和70N2号）的游离病毒基因组进行的结构分析表明，两种类型的游离基因的分子量均高于线性病毒粒子DNA。通过部分变性图谱研究了1670号游离基因中独特（L）和重复（H）DNA的排列。发现线性病毒粒子DNA中存在的部分L序列缺失，部分在游离基因中重复。游离L-DNA区域与线性HVS DNA的已知物理基因图谱相关。