Departamento de Química, Programa de Pós-graduação em Ciências Biológicas, Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas,Universidade Federal de Santa Maria, Cep 97105-900, Santa Maria, RS, Brazil.
Brain Res. 2012 Jan 9;1430:101-11. doi: 10.1016/j.brainres.2011.10.049. Epub 2011 Nov 4.
This study was designed to investigate the protective effects of the combination of guanosine and 2 organoselenium compounds (ebselen and diphenyl diselenide) against glutamate-induced oxidative stress in different regions of rat brains. Glutamate caused an increase in reactive oxygen species (ROS) generation and a decrease in [(3)H]-glutamate uptake in striatal, cortical, and hippocampal slices. Guanosine, ebselen, and diphenyl diselenide prevented glutamate-induced ROS production in striatal, cortical and hippocampal slices. The combination of guanosine with organoselenium compounds was more effective against glutamate-induced ROS production than the individual compounds alone. Guanosine prevented [(3)H]-glutamate uptake inhibition in striatal, cortical, and hippocampal slices. Thus, protection against the harmful effects of glutamate is possibly due to the combination of the antioxidant properties of organoselenium compounds and the stimulatory effect of guanosine on glutamate uptake. In conclusion, the combination of antioxidants and glutamatergic system modulators could be considered a potential therapy against the prooxidant effects of glutamate.
这项研究旨在探讨鸟苷与 2 种有机硒化合物（依布硒啉和二苯并二硒醚）联合使用对谷氨酸诱导的大鼠脑不同区域氧化应激的保护作用。谷氨酸可引起活性氧（ROS）生成增加和[（3）H] -谷氨酸摄取减少。鸟苷、依布硒啉和二苯并二硒醚可防止纹状体、皮质和海马切片中谷氨酸诱导的 ROS 产生。鸟苷与有机硒化合物的联合使用比单独使用单一化合物更能有效抑制谷氨酸诱导的 ROS 产生。鸟苷可防止纹状体、皮质和海马切片中[（3）H] -谷氨酸摄取抑制。因此，对抗谷氨酸有害影响的保护作用可能是由于有机硒化合物的抗氧化特性和鸟苷对谷氨酸摄取的刺激作用的结合。总之，抗氧化剂和谷氨酸能系统调节剂的联合使用可以被认为是一种对抗谷氨酸促氧化剂作用的潜在治疗方法。
