Department of Ophthalmology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
Oncol Rep. 2012 Mar;27(3):748-52. doi: 10.3892/or.2011.1565. Epub 2011 Nov 29.
The aim of this study was to investigate the effect of an adenosine A2A receptor antagonist on the expression of glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) in rat retinal Müller cells at elevated hydrostatic pressure in vitro. Immunofluorescence staining of GS and GFAP was used for the identification of Müller cells. The expression of GS and GLAST in different hydrostatic pressure (0, 20, 40, 60, 80 mmHg/24 h) was examined by real-time PCR and Western blotting to identify the most suitable pressure. Müller cells treated with 0.1, 1, 10 µM SCH 442416 (A2A receptor antagonist) in the most suitable pressure, and the levels of GS and GLAST were examined by real-time PCR and Western blotting. Significantly increased expression of GS and GLAST at 40 mmHg pressure was observed in Müller cells and treatment with 10 µM SCH 442416 in 40 mmHg pressure further promoted the expression of GS and GLAST. A2A receptor antagonist increased the expression of GLAST and GS of Müller cells and accelerated the clearance of extracellular glutamate.
本研究旨在探讨在体外高静压条件下，腺苷 A2A 受体拮抗剂对谷氨酸合成酶（GS）和谷氨酸-天门冬氨酸转运体（GLAST）在大鼠视网膜 Müller 细胞中表达的影响。通过免疫荧光染色鉴定 GS 和 GFAP，以鉴定 Müller 细胞。通过实时 PCR 和 Western blot 检测不同静压（0、20、40、60、80 mmHg/24 h）下 GS 和 GLAST 的表达，以确定最适压力。在最适压力下，用 0.1、1、10 µM SCH 442416（A2A 受体拮抗剂）处理 Müller 细胞，通过实时 PCR 和 Western blot 检测 GS 和 GLAST 的水平。结果发现，在 Müller 细胞中，40 mmHg 压力下 GS 和 GLAST 的表达明显增加，而在 40 mmHg 压力下用 10 µM SCH 442416 处理进一步促进了 GS 和 GLAST 的表达。A2A 受体拮抗剂增加了 Müller 细胞中 GLAST 和 GS 的表达，加速了细胞外谷氨酸的清除。
