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一种来自食药兼用的滑子菇（Hypsizygus marmoreus (Peck) Bigel.，蘑菇科）的抗真菌蛋白。

An antifungal protein from the culinary-medicinal beech mushroom, Hypsizygus marmoreus (Peck) Bigel. (Agaricomycetideae).

作者信息

Suzuki Tomohiro, Umehara Kanako, Tashiro Ai, Kobayashi Yuka, Dohra Hideo, Hirai Hirofumi, Kawagishi Hirokazu

机构信息

Innovation and Joint Research Center, Shizuoka University, Suruga-ku, Shizuoka, Japan.

出版信息

Int J Med Mushrooms. 2011;13(1):27-31. doi: 10.1615/intjmedmushr.v13.i1.40.

DOI:10.1615/intjmedmushr.v13.i1.40
PMID:22135901
Abstract

An antifungal protein (HM-af) was purified from the culinary-medicinal mushroom Hypsizygus marmoreus. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry of HM-af indicated that its molecular mass was 9.5 kDa. The N-terminal amino acid sequence of HM-af showed homology to ribonuclease H from Clostridium thermocellum. HM-af showed the antifungal activity against Flammulina velutipes.

摘要

从食药用真菌玉蕈中纯化出一种抗真菌蛋白（HM-af）。HM-af的十二烷基硫酸钠聚丙烯酰胺凝胶电泳和基质辅助激光解吸电离飞行时间质谱结果表明，其分子量为9.5 kDa。HM-af的N端氨基酸序列与嗜热栖热放线菌的核糖核酸酶H具有同源性。HM-af对金针菇表现出抗真菌活性。

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