肺泡菌素，一种源自野生蘑菇肺泡多孔菌的新型抗真菌多肽。

Alveolarin, a novel antifungal polypeptide from the wild mushroom Polyporus alveolaris.

作者信息

Wang Hexiang, Ng T B, Liu Qinghong

机构信息

State Key Laboratory of Agrobiotechnology, Beijing and Department of Microbiology, College of Biological Science, Chain Agricultural University, Beijing, China.

出版信息

Peptides. 2004 Apr;25(4):693-6. doi: 10.1016/j.peptides.2004.01.026.

DOI:10.1016/j.peptides.2004.01.026

PMID:15165727

Abstract

An antifungal polypeptide, with a molecular mass of 28 kDa as judged by gel filtration and appearing as a single band with a molecular mass of 14 kDa in sodium dodecyl suflate-polyacrylamide gel electrophoresis, was isolated from fresh fruiting bodies of the mushroom Polyporus alveolaris. The antifungal polypeptide, designated as alveolarin, demonstrated an inhibitory action on mycelial growth in Botrytis cinerea, Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. Alveolarin was isolated with a procedure that entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration on Superdex 75 by fast protein liquid chromatography.

摘要

从肺泡多孔菌新鲜子实体中分离出一种抗真菌多肽。通过凝胶过滤法判断其分子量为28 kDa，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中呈现为一条分子量为14 kDa的单带。这种抗真菌多肽被命名为肺泡菌素，它对灰葡萄孢、尖孢镰刀菌、花生球腔菌和梨孢盘菌的菌丝生长具有抑制作用。肺泡菌素是通过以下步骤分离得到的：在DEAE-纤维素上进行离子交换色谱，在Affi-凝胶蓝胶上进行亲和色谱，以及通过快速蛋白质液相色谱在Superdex 75上进行凝胶过滤。